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electrodes  (KCAS Bioanalytical and Biomarker Services)
94
KCAS Bioanalytical and Biomarker Services electrodes
Electrodes, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrodes/product/KCAS Bioanalytical and Biomarker Services
Average 94 stars, based on 1 article reviews
electrodes - by Bioz Stars, 2026-03
94/100 stars
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skov-3-gfp-luc (rrid : cvcl_jy94)  (Cell Biolabs Inc)
90
Cell Biolabs Inc skov-3-gfp-luc (rrid : cvcl_jy94)
NK cells maintain cytotoxicity against tumor cells after cryopreservation. PM21-NK cells were expanded from T-cell-depleted PBMCs obtained from multiple donors (N = 2–3). Cells were cryopreserved while donor-matched fresh PM21-NK cells (black squares) were maintained in culture. Frozen NK cells were thawed and rested 16 h the day before analysis (blue triangles) or thawed and rested 1 h on the day of analysis (gray circles). Fresh or thawed NK cells were added to K562-GFPLuc cells at the indicated NK:K562 ratio (A) or at a ratio of 1:1 (B) and co-incubated for 60 min to measure cytotoxicity using Annexin V assay. Cryopreserved PM21-NK cells were less cytotoxic against K562 cells 1 h post-thaw compared to fresh PM21-NK cells but recovered cytotoxicity when rested 16 h post-thaw (N = 3 donors). (A, B) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed <t>SKOV-3</t> cells similarly or better compared to matched fresh PM21-NK cells (black squares) (one representative donor in triplicate) (C) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed SKOV-3 cells similarly or better compared to matched fresh PM21-NK cells (black squares) at a 1:1 NK : SKOV-3 ratio (N = 2 in triplicate). (D) Concentration-dependent cytotoxicity curves were generated by measuring cytotoxicity at the indicated time-points of the cytotoxicity assay at multiple NK : SKOV-3 cell ratios (E) . Cryopreserved PM21-NK cells rested for 16 h post-thaw had comparable or increased cytotoxicity compared to donor and expansion-matched fresh PM21-NK cells (N = 2 in triplicate) (E) . Data are presented as scatter plots or bar graphs with error bars representing standard deviation. Statistical significance was determined by multiple paired t-tests. For cytotoxicity plots, the area under the curve was measured and then statistical significance was determined by unpaired t-tests. p values are shown as *p < 0.05, **p < 0.01.
Skov 3 Gfp Luc (Rrid : Cvcl Jy94), supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/skov-3-gfp-luc (rrid : cvcl_jy94)/product/Cell Biolabs Inc
Average 90 stars, based on 1 article reviews
skov-3-gfp-luc (rrid : cvcl_jy94) - by Bioz Stars, 2026-03
90/100 stars
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NK cells maintain cytotoxicity against tumor cells after cryopreservation. PM21-NK cells were expanded from T-cell-depleted PBMCs obtained from multiple donors (N = 2–3). Cells were cryopreserved while donor-matched fresh PM21-NK cells (black squares) were maintained in culture. Frozen NK cells were thawed and rested 16 h the day before analysis (blue triangles) or thawed and rested 1 h on the day of analysis (gray circles). Fresh or thawed NK cells were added to K562-GFPLuc cells at the indicated NK:K562 ratio (A) or at a ratio of 1:1 (B) and co-incubated for 60 min to measure cytotoxicity using Annexin V assay. Cryopreserved PM21-NK cells were less cytotoxic against K562 cells 1 h post-thaw compared to fresh PM21-NK cells but recovered cytotoxicity when rested 16 h post-thaw (N = 3 donors). (A, B) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed SKOV-3 cells similarly or better compared to matched fresh PM21-NK cells (black squares) (one representative donor in triplicate) (C) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed SKOV-3 cells similarly or better compared to matched fresh PM21-NK cells (black squares) at a 1:1 NK : SKOV-3 ratio (N = 2 in triplicate). (D) Concentration-dependent cytotoxicity curves were generated by measuring cytotoxicity at the indicated time-points of the cytotoxicity assay at multiple NK : SKOV-3 cell ratios (E) . Cryopreserved PM21-NK cells rested for 16 h post-thaw had comparable or increased cytotoxicity compared to donor and expansion-matched fresh PM21-NK cells (N = 2 in triplicate) (E) . Data are presented as scatter plots or bar graphs with error bars representing standard deviation. Statistical significance was determined by multiple paired t-tests. For cytotoxicity plots, the area under the curve was measured and then statistical significance was determined by unpaired t-tests. p values are shown as *p < 0.05, **p < 0.01.

Journal: Frontiers in Immunology

Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo

doi: 10.3389/fimmu.2022.861681

Figure Lengend Snippet: NK cells maintain cytotoxicity against tumor cells after cryopreservation. PM21-NK cells were expanded from T-cell-depleted PBMCs obtained from multiple donors (N = 2–3). Cells were cryopreserved while donor-matched fresh PM21-NK cells (black squares) were maintained in culture. Frozen NK cells were thawed and rested 16 h the day before analysis (blue triangles) or thawed and rested 1 h on the day of analysis (gray circles). Fresh or thawed NK cells were added to K562-GFPLuc cells at the indicated NK:K562 ratio (A) or at a ratio of 1:1 (B) and co-incubated for 60 min to measure cytotoxicity using Annexin V assay. Cryopreserved PM21-NK cells were less cytotoxic against K562 cells 1 h post-thaw compared to fresh PM21-NK cells but recovered cytotoxicity when rested 16 h post-thaw (N = 3 donors). (A, B) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed SKOV-3 cells similarly or better compared to matched fresh PM21-NK cells (black squares) (one representative donor in triplicate) (C) . Cryopreserved PM21-NK cells that were rested 16 h post-thaw (blue triangles), killed SKOV-3 cells similarly or better compared to matched fresh PM21-NK cells (black squares) at a 1:1 NK : SKOV-3 ratio (N = 2 in triplicate). (D) Concentration-dependent cytotoxicity curves were generated by measuring cytotoxicity at the indicated time-points of the cytotoxicity assay at multiple NK : SKOV-3 cell ratios (E) . Cryopreserved PM21-NK cells rested for 16 h post-thaw had comparable or increased cytotoxicity compared to donor and expansion-matched fresh PM21-NK cells (N = 2 in triplicate) (E) . Data are presented as scatter plots or bar graphs with error bars representing standard deviation. Statistical significance was determined by multiple paired t-tests. For cytotoxicity plots, the area under the curve was measured and then statistical significance was determined by unpaired t-tests. p values are shown as *p < 0.05, **p < 0.01.

Article Snippet: For mouse experiments, SKOV-3-GFP-Luc (RRID : CVCL_JY94) from Cell Biolabs, Inc. (San Diego, CA, USA), were passaged once through NSG mice to increase their tumorigenicity and sorted based on GFP expression.

Techniques: Incubation, Annexin V Assay, Concentration Assay, Generated, Cytotoxicity Assay, Standard Deviation

Cryopreserved NK cells maintain cytotoxicity in 3D tumor cell spheroid models. PM21-NK cells were expanded from T-cell-depleted PBMCs obtained from two donors. Cells were cryopreserved while donor-matched fresh PM21-NK cells were maintained in culture. Frozen NK cells were thawed and rested 16 h the day before analysis. NK-cell cytotoxicity was determined by kinetic live-cell imaging assay. Cryopreserved PM21-NK cells (blue triangles) were as efficacious at killing as fresh donor and expansion-matched PM21-NK cells (black squares) when 3,300 NK cells were cocultured with A549 spheroids (A) or SKOV-3 spheroids (D) (one representative donor in triplicate). Concentration-dependent cytotoxicity curves were also generated, and no significant difference between cryopreserved PM21-NK cells or fresh PM21-NK cells was found after 48 h of coculture with A549 spheroids (B) or SKOV-3 spheroids (E) (one representative donor in triplicate). Cryopreserved PM21-NK cells killed similarly A549 (C) or SKOV-3 (F) spheroids compared to matched fresh PM21-NK cells (3,300 NK/spheroid, N = 2 donors, in triplicate). Data are presented as scatter plots or bar graphs with error bars representing standard deviation. No statistical significance was determined by two-way ANOVA or multiple t-tests. For cytotoxicity time course plots, the area under the curve was measured and then statistical significance was determined by unpaired t-tests.

Journal: Frontiers in Immunology

Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo

doi: 10.3389/fimmu.2022.861681

Figure Lengend Snippet: Cryopreserved NK cells maintain cytotoxicity in 3D tumor cell spheroid models. PM21-NK cells were expanded from T-cell-depleted PBMCs obtained from two donors. Cells were cryopreserved while donor-matched fresh PM21-NK cells were maintained in culture. Frozen NK cells were thawed and rested 16 h the day before analysis. NK-cell cytotoxicity was determined by kinetic live-cell imaging assay. Cryopreserved PM21-NK cells (blue triangles) were as efficacious at killing as fresh donor and expansion-matched PM21-NK cells (black squares) when 3,300 NK cells were cocultured with A549 spheroids (A) or SKOV-3 spheroids (D) (one representative donor in triplicate). Concentration-dependent cytotoxicity curves were also generated, and no significant difference between cryopreserved PM21-NK cells or fresh PM21-NK cells was found after 48 h of coculture with A549 spheroids (B) or SKOV-3 spheroids (E) (one representative donor in triplicate). Cryopreserved PM21-NK cells killed similarly A549 (C) or SKOV-3 (F) spheroids compared to matched fresh PM21-NK cells (3,300 NK/spheroid, N = 2 donors, in triplicate). Data are presented as scatter plots or bar graphs with error bars representing standard deviation. No statistical significance was determined by two-way ANOVA or multiple t-tests. For cytotoxicity time course plots, the area under the curve was measured and then statistical significance was determined by unpaired t-tests.

Article Snippet: For mouse experiments, SKOV-3-GFP-Luc (RRID : CVCL_JY94) from Cell Biolabs, Inc. (San Diego, CA, USA), were passaged once through NSG mice to increase their tumorigenicity and sorted based on GFP expression.

Techniques: Live Cell Imaging, Concentration Assay, Generated, Standard Deviation

Cryopreserved NK cells maintain antitumor effect in an in vivo mouse model. 1 × 10 6 SKOV-3-GFP-luc cells were injected to the intraperitoneal ( i.p. ) cavity of NSG (NOD-scid IL-2Rγnull) female mice and allowed to seed for 4 days. Mice were then treated with 2 doses of either fresh or cryopreserved NK cells that were thawed and directly injected i.p. along with IL-2 (25,000 U, 3×/week). Mice were imaged 26 days after the first NK cell injection using In-Vivo Xtreme II imager and checked periodically for health status. A schematic of the experiment is shown (A) . Day 26 in vivo images (B) and quantification of luminescence (C) showed that mice treated with either frozen NK cells (triangles) or fresh NK cells (circles) had lower and comparable tumor burden based on the luminescence quantification compared to the signal measured in the only remaining untreated mouse (square). Mice injected with frozen NK cells (red dotted line) or fresh NK cells (blue dashed line) had a similar significant increase in survival compared to untreated mice (black solid line). Statistical significance was determined by Survival Curve comparison using the Gehan–Breslow–Wilcoxon test.

Journal: Frontiers in Immunology

Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo

doi: 10.3389/fimmu.2022.861681

Figure Lengend Snippet: Cryopreserved NK cells maintain antitumor effect in an in vivo mouse model. 1 × 10 6 SKOV-3-GFP-luc cells were injected to the intraperitoneal ( i.p. ) cavity of NSG (NOD-scid IL-2Rγnull) female mice and allowed to seed for 4 days. Mice were then treated with 2 doses of either fresh or cryopreserved NK cells that were thawed and directly injected i.p. along with IL-2 (25,000 U, 3×/week). Mice were imaged 26 days after the first NK cell injection using In-Vivo Xtreme II imager and checked periodically for health status. A schematic of the experiment is shown (A) . Day 26 in vivo images (B) and quantification of luminescence (C) showed that mice treated with either frozen NK cells (triangles) or fresh NK cells (circles) had lower and comparable tumor burden based on the luminescence quantification compared to the signal measured in the only remaining untreated mouse (square). Mice injected with frozen NK cells (red dotted line) or fresh NK cells (blue dashed line) had a similar significant increase in survival compared to untreated mice (black solid line). Statistical significance was determined by Survival Curve comparison using the Gehan–Breslow–Wilcoxon test.

Article Snippet: For mouse experiments, SKOV-3-GFP-Luc (RRID : CVCL_JY94) from Cell Biolabs, Inc. (San Diego, CA, USA), were passaged once through NSG mice to increase their tumorigenicity and sorted based on GFP expression.

Techniques: In Vivo, Injection